TEST METHOD FOR TOTAL CHLORINE IN INDUSTRIAL WATER (MR: 1-200, HR: 10-200, 100-2000 & above mg/L as Cl2)
BACKGROUND OF THE TEST:
There are numerous applications where chlorine is dosed
as disinfectant because it is a strong oxidizing agent and is
considered as one of the most powerful and economical
disinfectant at all. Consequently, chlorine residuals are
maintained in water based on a specific application.
Nuclotrasis offers user friendly test method with ease of
handling the relevant reagents and other equipment in
which the sample is first acidified and hence titrated under
acidic conditions by an appropriate reducing agent until the
color changes to colorless The test ideally covers the range
as: MR: 1-200, HR: 10-200, 100-2000 & above mg/L as Cl2.
REAGENTS/EQUIPMENTS/APPARATUS:
• Provided 5 mL & 10 mL marked glass vial (Item
Code: 02-01)
• Provided MS spatula or say scoop
• NUCLOTRASIS TOTAL CHLORINE BUFFER SOLUTION
(Item Code: 01-11-1)
• NUCLOTRASIS TOTAL CHLORINE TEST REAGENT
(Item Code: 01-11-2)
• NUCLOTRASIS TOTAL CHLORINE INDICATOR
SOLUTION (Item Code: 01-11-3)
• NUCLOTRASIS TOTAL CHLORINE MR-TITRANT
SOLUTION (Item Code: 01-11-4)
• NUCLOTRASIS TOTAL CHLORINE HR-TITRANT
SOLUTION (Item Code: 01-11-5)
SAMPLE COLLECTION & PREPARATION
• Make sure to immediately analyze the sample
after collection of sample.
• Ensure to collect sample in a suitable and
designated bottle for a specific sample.
• Thoroughly rinse the sample bottle with
current sample and fill the sample by allowing
slight overflow and immediately tighten the
cap.
TEST PROCEDURE:
1. We recommend selecting the sample volume in
the provided glass vial (Item Code: 02-01)
based on your expected/desired total chlorine
residuals from medium range (MR) and high
range (HR) tables below.
Table-I: For Total Chlorine MR (1-200 & above ppm)
Range
(mg/L as Cl2)
Sample volume
(mL)
Multiplication
Factor
1-20 10 1
10-40 5 2
10-100 2 5
10-200 and above 1 10
2 After required volume of sample as per
aforementioned tables, is taken, dilute it to 50 mL
with deionized water then add 10 drops of
NUCLOTRASIS TOTAL CHLORINE BUFFER
SOLUTION (Item Code: 01-11-1) and swirl to mix.
3 Then add 1 full scoop (almost 1 gm) of
NUCLOTRASIS TOTAL CHLORINE TEST REAGENT
(Item Code: 01-11-2) through provided spatula
and swirl to mix.
4 Now start adding NUCLOTRASIS Total Chlorine
MR or HR Titrant Solution (Item Code: 01-11-4)
drop wise with gentle mixing and while counting
the number of drops added until the pale yellow
color is obtained.
5 Now add 5 drops of TOTAL CHLORINE
INDICATOR SOLUTION (Item Code: 01-11-3).
Consequently, dark blue color solution will
appear.
6 Now again start adding NUCLOTRASIS Total
Chlorine MR or HR Titrant Solution (Item Code:
01-11-4) drop wise with gentle mixing and while
continue the counting with previously counted
number of drops until colorless solution is
obtained.
7 Note the total number of drops added and
multiply with multiplication factor mentioned in
either table-I or table-II based on selected sample
range and volume.
8 Report the final reading as required by the
system.
REFERENCES:
1. Standard Methods for the Examination of Water
and Wastewater
Table-II: For Total Chlorine HR (10-2000 & above ppm)
Range
(mg/L as Cl2)
Sample volume
(mL)
Multiplication
Factor
10-200 10 10
20-400 5 20
50-1000 2 50
100-2000 and above 1 100