TEST METHOD FOR TOTAL IRON IN INDUSTRIAL WATER (RANGE: 0-4.9 mg/L Fe)
BACKGROUND OF THE TEST:
In industrial applications, presence of iron ions in water is considered to be highly objectionable
such as for food & beverages, agricultural applications, industrial pipelines regarding corrosion,
swimming pools etc making the determination of iron as in important test.
Nuclotrasis offers user friendly test method with ease of handling the relevant reagents and other
equipments used in the analysis. For determination of total iron in medium range zone, this test
relies on Lambert Bear’s Law of spectrometry where in a mixture of Fe+2 & Fe+3 iron ions in the
sample, all Fe+3 ions are also reduced to Fe+2 state through a suitable reducing agent. Then, all Fe+2
ions are reacted with 1-10 Ortho Phenanthroline indicator to form an orange color complex.
Intensity of the orange color is proportional to the concentration of total iron in a particular sample.
Measurement wavelength is 510 nm for spectrophotometers or 520 nm for colorimeters.
REAGENTS/EQUIPMENTS/APPARATUS:
Any Suitable Spectrophotometer
Relevant Cuvette or cell of Photometer
NUCLOTRASIS IRON-I TEST SOLUTION (Item Code: 01-04-1)
NUCLOTRASIS IRON-II TEST REAGENT (Item Code: 01-04-2)
NUCLOTRASIS IRON-II TEST SOLUTION (Item Code: 01-04-3)
SAMPLE COLLECTION & PREPARATION
Make sure to immediately analyze the sample after collection of sample.
Ensure to collect sample in a suitable and designated bottle for a specific sample.
Ensure to conduct this test at room temperatures.
TEST PROCEDURE:
1. Take 10 mL sample in a cuvette or any other test tube and call it as “A”.
2. Similarly take 10 mL deionized water in another cuvette or test tube for reagent blank and
call it as “B”.
3. In the sample, add 10 drops of NUCLOTRASIS IRON-I TEST SOLUTION (Item Code: 01-04-1)
and mix gently.
4. Then add almost 0.2 gm of NUCLOTRASIS IRON-II TEST REAGENT (Item Code: 01-04-2)
spatula provided with the kit in the sample and mix gently.
5. Now add 5 drops of NUCLOTRASIS IRON-III TEST SOLUTION (Item Code: 01-04-3) and mix
gently. Now allow to stand for 10 minutes.
6. Meanwhile, select Iron MR or Iron specific channel/test in the spectrophotometer.
7. First run reagent blank “B” to achieve blanking.
8. Then run test sample “A” and note the Photometer display reading in µg/L or mg/L.
9. Multiply the reading with dilution factor 1.075 as dilution factor.
10. Report the reading as required by the system.
REFERENCES:
Standard Methods for the Examination of Water and Wastewater