TEST METHOD FOR HYDRAZINE IN INDUSTRIAL WATER (RANGE: 0-500 µg/L or say 0-0.5 mg/L N2H4)
BACKGROUND OF THE TEST:
Hydrazine having chemical formula as N2H4 has numerous applications such as oxygen scavenger to
cater corrosion attack in the boilers circuits, for synthesis of various pesticides, in rockets fuels, etc.
Nuclotrasis offers user friendly test method with ease of handling the relevant reagents and other
equipments used in the analysis. For determination of hydrazine, this test relies on Lambert Bear’s
Law of spectrometry where para-dimethylaminobenzaldehyde in acidic medium is allowed to react
with hydrazine developing yellow color complex that is directly proportional to the hydrazine
concentration in the solution. In this test, wavelength for maximum absorbance is 455 nm for
spectrophotometers and 420 nm for colorimeters.
REAGENTS/EQUIPMENTS/APPARATUS:
Provided 10 mL marked glass vial (Item Code: 02-01)
Any Suitable Spectrophotometer or available softcopy of color comparative chart
Relevant Cuvette or cell of Photometer
NUCLOTRASIS HYDRAZINE TEST SOLUTION (Item Code: 01-01)
SAMPLE COLLECTION & PREPARATION
Make sure to immediately analyze the sample after collection of sample.
Ensure to collect sample in a suitable and designated bottle for a specific sample.
Thoroughly rinse the sample bottle with current sample and fill the sample by allowing
slight overflow and immediately tighten the cap.
Prevent agitation of the sample and exposure to air as the test is sensitive to the air.
TEST PROCEDURE:
1. Take sample to 10 mL mark into the provided vial (Item Code: 02-01) and call it as “A”.
2. Similarly take 10 mL deionized water in another cuvette or test tube for reagent blank and
call it as “B”.
3. In the sample, add 20 drops (1 mL) NUCLOTRASIS HYDRAZINE TEST SOLUTION and mix
gently.
4. Allow to stand for ten (10) minutes for full color development.
5. Meanwhile, select Hydrazine specific channel/test in the spectrophotometer.
6. First run reagent blank “B” to achieve blanking.
7. Then run test sample “A” and note the Photometer display reading in µg/L or mg/L. In case
photometer is not available, match the developed color with the provided soft copy color
comparator of this HYDRAZINE test kit.
8. Multiply the reading with dilution factor 1.1.
REFERENCES: ASTM Manual of Industrial Water, D1385-78, 376 (1979)